HLA Binding Assays

HLA Binding Assays

Class II: Class II HLA binding assays may be used to measure affinity of putative epitopes for multiple HLA alleles. EpiVax employs a competition-based HLA binding assay originally described by Steere et al. [1] and adapted for commercial use by EpiVax. Our assay employs highly-purified Class II molecules for which we have an exclusive worldwide license. This assay format is far superior in sensitivity and specificity compared to cell-based assay formats. Based on a 5-7 concentration peptide titration, non-linear regression analysis is performed and an IC50 value is calculated. Binding assays are performed for four to eight alleles including: DRB1*0101, DRB1*0301, DRB1*0401, DRB1*0701, DRB1*0801, DRB1*1101, DRB1*1301 and DRB1*1501.
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[1] Assay described in: Steere AC, Klitz W, Drouin EE, Falk BA, Kwok WW, Nepom GT, Baxter-Lowe LA. Antibiotic-refractory Lyme arthritis is associated with HLA-DR molecules that bind a Borrelia burgdorferi peptide. J Exp Med. 2006 Apr 17;203(4):961-71.

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