HLA Transgenic Mice

EpiVax maintains human leukocyte antigen (HLA) DR3 transgenic mouse colonies and has access to DR4 transgenic mouse colonies to assess immunogenicity of vaccines and protein therapeutics in the context of a human-like antigen-presentation system.

HLA transgenic mouse colonies are maintained at Taconic Farms and where these mice are housed in pathogen-free isolators. Sentinel mice are tested regularly for a panel of murine bacterial and viral infectious agents. Transgenic mice are genotyped by standard PCR using genomic DNA extracted from tail tissue and primer pairs specific for HLA transgenes. HLA expression levels are assessed by flow cytometry using mouse peripheral blood mononuclear cells immunostained for HLA. Available assay formats include:

  • Serum antibody levels by ELISA,
  • T cell cytokine expression (ELISpot and multiplex ELISA)
  • T cell phenotype using flow cytometry and cytotoxic T cell activity

Email Us Here for a PDF brochure describing our Lab-Based Services and/or a Cost Estimate for your project.

This is an example of an HLA-transgenic mouse
response to a human protein therapeutic.

EpiVax maintains human leukocyte antigen
(HLA) DR3 and DR4 transgenic mice.

We are dedicated to applying our tools (in-silico, in-vitro and in-vivo) to re-engineering therapeutic proteins and to designing new vaccines. For a recent interview highlighting our CEO’s latest ideas, click on the video: